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By Jon A. Van Loon

Analytical Atomic Absorption Spectroscopy

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A c o r r e c t i o n to the a b s o r p t i o n m u s t b e m a d e by subtracting the background. The two approaches most c o m m o n l y u s e d to o v e r c o m e this p r o b l e m a r e the n o n a b s o r b i n g - l i n e a n d t h e continuum methods. 0 Nonabsorbing-Line Method Emission from hollow-cathode lamps contains not only resonance l i n e s of t h e e l e m e n t of i n t e r e s t , b u t f r e q u e n t l y n o n a b s o r b i n g lines of this e l e m e n t a n d lines resulting f r o m e x c i t a t i o n of t h e filler g a s .

C o r r e c t i o n for nonspecific a b s o r p t i o n is d e s c r i b e d o n p. 4 4 . It is i m p o r t a n t to test the n e e d for b a c k g r o u n d c o r r e c t i o n in all trace m e t a l analyses w h e n u s i n g e l e c t r o t h e r m a l a t o m i z e r s a n d w i t h flames w h e n w o r k i n g n e a r t h e d e t e c t i o n limit. In t h e c a s e of the f o r m e r b a c k g r o u n d , c o r r e c t i o n is a l m o s t a l w a y s n e c e s s a r y . Chemical Interferences D u r i n g the early y e a r s of a t o m i c a b s o r p t i o n m a n y w o r k e r s failed to recognize the serious problems caused by chemical interference.

In o t h e r c a s e s , c o m b u s t i b l e releasing a g e n t s s u c h as E D T A c o m b i n e p r e f e r e n ­ tially w i t h t h e a n a l y t e a t o m a n d t h e n b u r n in t h e f l a m e - r e l e a s i n g a n a ­ lyte a t o m s . In the c a l c i u m e x a m p l e , t h e p h o s p h a t e i n t e r f e r e n c e c a n b e alleviated b y e i t h e r a d d i n g a releasing a g e n t or b y u s i n g the h i g h e r - t e m p e r a t u r e n i t r o u s o x i d e - a c e t y l e n e flame.

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